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The Museum is located in the former crypt of a late 19th century church, which has been extensively restored. A guided tour is advised for student groups and a recommended visit is 1 hour.

The Museum’s three sections focus on the healthcare of the 18th, 19th and 20th centuries and include history of the hospital since its foundation in 1740, Joseph Merrick (the 'Elephant Man'), the Whitechapel murders, and former London hospital nurses Edith Cavell and Eva Luckes.

It also has a permanent exhibition of artefacts and archives relating to the hospital and the history of healthcare in the East End. Works of art, surgical instruments, medical and nursing equipment, uniforms, medals, and written archives and printed books are included.